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| 鱼油中EPA和DHA的酶法富集工艺研究 |
| Enzymatic enrichment process of EPA and DHA in fish oi |
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| DOI： |
| 中文关键词: 精制鱼油 Sn-1,3特异性脂肪酶 富集 EPA DHA |
| 英文关键词:refined fish oil Sn-1,3 special lipase enrichment EPA DHA |
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| 中文摘要: |
| 利用Sn-1,3特异性脂肪酶对食品级鱼油中的二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）进行富集，而后利用偏甘油酯脂肪酶除去上述步骤产生的甘油二酯、甘油一酯，后处理得到具有天然属性的精制鱼油。采用单因素实验考察特异性脂肪酶富集EPA和DHA过程中脂肪酶用量、纯化水加入量、水解时间及水解温度对富集EPA和DHA的影响。运用气相色谱分析精制鱼油的脂肪酸组成。结果表明：特异性脂肪酶富集过程的最优工艺条件为在pH 6条件下固定化Sn-1,3特异性脂肪酶用量1%~15%、纯化水加入量1%~10%、水解时间2~6 h、水解温度30~70?℃，富集后EPA和DHA总量为40%~60%，产品收率在48%~75%；精制鱼油含有21种主要脂肪酸，其中EPA和DHA含量最多。 |
| 英文摘要: |
| The eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) of food grade fish oil were enriched by Sn-1,3 specific lipase, and then the diacylglycerol and glycerin monoester, which were produced from the above step, were removed by partial glyceride lipase. The refined fish oil with nature properties was produced after post-processing. The influences of specific lipase dosage, purified water dosage, hydrolysis time and hydrolysis temperature on total content of EPA and DHA were investigated by single factor experiment. The fatty acid composition of refined fish oil was analyzed by gas chromatography. The results showed that the optimal enrichment conditions of EPA and DHA were obtained as follows: pH 6, immobilized Sn-1,3 specific lipase dosage 1%-15%, purified water dosage 1%-10%, hydrolysis time 2-6 h and hydrolysis temperature 30-70?℃. After enrichment, the total content of EPA and DHA was 40%-60%, and the yield of the product was 48%-75%. Refined fish oil contained 21 kinds of main fatty acids, in which the contents of EPA and DHA were the highest. |
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