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| 新型液态固定化脂肪酶制备及其稳定性的研究 |
| Preparation of novel liquid immobilized lipase and its stability |
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| DOI： |
| 中文关键词: 三液相体系 液态固定化载体 脂肪酶 固定化酶 |
| 英文关键词:three liquid phase system liquid immobilized carrier lipase immobilized enzyme |
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| 中文摘要: |
| 传统的固态固定化脂肪酶存在载体易破碎、制备成本高等问题，制约了其工业应用。利用前期开发的三液相体系中相作为脂肪酶液态固定化载体，研究了不同种类的成相物质、成相物质的比例以及不同种类的脂肪酶对液态固定化酶回收率的影响。同时考察了该液态固定化载体固定脂肪酶的稳定性。结果表明：正己烷/PEG400/Na2SO4三液相体系中相为更优的液态固定化酶载体；最优的制备条件为PEG400比例20%、Na2SO4比例14%，此条件下AY30脂肪酶的回收率可达98%以上，其他类型脂肪酶中相回收率均大于90%；该液态固定化载体在30?℃的环境下能有效保持脂肪酶的催化活性，保护效果在不同温度下均明显优于传统水-有机溶剂体系；同时，脂肪酶在此液态固定化载体中的保藏稳定性也优于水环境，低温保藏2周后仍能保留约78.4%的酶活力，体现出较好的保藏稳定性。 |
| 英文摘要: |
| Traditional solid immobilized lipase has problems such as fragile carrier, high preparation cost etc., which restricts its industrial application. Three liquid phase system developed in the previous period was used as a liquid immobilized carrier for lipase.The effects of different kinds of phase-forming substances, the proportion of phase-forming substances and different kinds of lipases on the recovery of immobilized liquid enzyme were studied. And the stability of the lipase immobilized on the liquid immobilized carrier was investigated. The results showed that the middle phase of n-hexane/PEG400/Na2SO4 system was a better liquid immobilized lipase carrier. The optimal conditions were obtained as follows: PEG400 ratio 20%, and Na2SO4 ratio 14%. Under these conditions, the recovery of lipase AY30 could be over 98%, and the recoveries of other types of lipases were all more than 90%. The liquid immobilized carrier could effectively maintain the catalytic activity of the lipase at 30?℃, and the protective effect was obviously superior to the traditional water-organic solvent system at different temperatures. At the same time, the preservation stability of the lipase in the liquid immobilized carrier was also better than that in the water environment. After two weeks of low temperature preservation, it could still retain about 78.4% of the enzyme activity, which showed good preservation stability. |
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