| Taking Zanthoxylum armatum seed as the material, the physicochemical properties including the basic chemical composition, protein isoelectric point, amino acid composition, subunit composition and protein component of the defatted Zanthoxylum armatum seed shell and kernel were mainly studied. The results showed that the protein contents of the defatted Zanthoxylum armatum seed shell and kernel were 61.17% and 854%, and the protein isoelectric points were 3.5 and 4.5, respectively. The total amino acid content of the defatted seed kernel powder was 43 g/100 g, and its essential amino acid accounted for 2731% of the total amount. The total amino acid content of the defatted seed shell powder was 4.07 g/100 g, and its essential amino acid accounted for 26.78% of the total amount. The main amino acids of the defatted seed shell and kernel were aspartic acid, glutamic acid and arginine. The protein was classified by Osborne method, and the contents of albumin, globulin, gluten and gliadin in defatted seed kernel were 776%, 72.06%, 15.80% and 1.22%, respectively. The corresponding protein contents in defatted seed shell were 13.12%, 56.54%, 21.45% and 3.25%, respectively. SDS-PAGE analysis showed that there were seven bands of crude protein in defatted seed kernel, and the relative molecular weight ranged from 0 to 66.200 kDa; the crude protein in defatted seed shell had two bands, and the relative molecular weight was 18400-35.000 kDa. The relative molecular weights of four protein components in seed kernel were small, and the relative molecular weight distribution of their subunits was 0-45 kDa. The distribution of the four protein components of the defatted seed shell was small, and the relative molecular weight distribution of their subunits was 14.4-35 kDa. |
