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| 油茶蒲不同溶剂粗提液的总黄酮提取率与抗氧化活性比较 |
| Total flavonoids extraction rate and antioxidant activities of crude extracts from Camellia oleifera fruit hull by different solvents |
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| DOI： |
| 中文关键词: 油茶蒲 粗提液 黄酮 抗氧化活性 |
| 英文关键词:Camellia oleifera fruit hull crude extract flavonoids antioxidant activity |
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| 中文摘要: |
| 为探索油茶蒲的有效利用途径，采用各具5个体积分数梯度的5种有机溶剂对油茶蒲进行超声辅助浸提而得到25种粗提液，研究比较了各粗提液的总黄酮提取率和清除DPPH·的能力。结果表明：总黄酮提取率最高的5种溶剂及其对应的总黄酮提取率依次是60%丙酮溶液（6.28%）、60%乙醇溶液（5.24%）、80%甲醇溶液（4.82%）、60%1,3-丁二醇溶液（4.40%）、 60%乙酸乙酯-乙醇溶液（4.13%）；清除DPPH·能力最强的5种粗提液及其对应的清除率依次是100%乙醇溶液提取物（92.78%）、60%乙酸乙酯-乙醇溶液提取物（92.69%）、100%1,3-丁二醇溶液提取物（89.68%）、100%甲醇溶液提取物（86.54%）、100%丙酮溶液提取物（81.99%）。黄酮提取率最高的油茶蒲提取物所对应的DPPH·清除能力并不是最强的，说明油茶蒲提取物中除了黄酮以外，可能还有多酚、皂素、多糖等其他抗氧化活性成分。适合油茶蒲黄酮利用的最佳提取剂是60%乙酸乙酯-乙醇溶液，总黄酮提取率达4.13%，所对应提取物对DPPH·清除率达92.69%，是一种适应工业化开发利用油茶蒲黄酮的理想溶剂。 |
| 英文摘要: |
| In order to explore the effective utilization ways of Camellia oleifera fruit hull, 25 crude extracts were obtained by ultrasound-assisted extraction of Camellia oleifera fruit hull of five organic solvents with five volume fraction gradients respectively, and the extraction rates of total flavonoids and the abilities of scavenging DPPH free radical of various crude extracts were compared. The results showed that five solvents with the highest extraction rates of total flavonoids and their corresponding extraction rate of total flavonoids were obtained as follows: 60% acetone solution(6.28%), 60% ethanol solution(5.24%), 80% methanol solution(4.82%), 60%1,3-butanediol solution(4.40%), 60% ethyl acetate - ethanol solution(4.13%). The five crude extracts with the strongest scavenging ability of DPPH free radical and their corresponding scavenging rates were obtained as follows: 100% ethanol extract(92.78%), 60% ethyl acetate-ethanol solution extract(92.69%), 100% 1,3-butanediol extract(89.68%), 100% methanol extract(86.54%) and 100% acetone extract (81.99%). The DPPH free radical scavenging ability of the crude extract of Camellia oleifera fruit hull with the highest extraction rate of total flavonoids was not the strongest, which indicated that besides flavonoids, the crude extracts of Camellia oleifera fruit hull also contained other antioxidative components, such as polyphenols, saponins and polysaccharides. The optimal extractant for effective utilization of flavonoids in Camellia oleifera fruit hull was 60% ethyl acetate-ethanol solution, the total flavonoids extraction rate and the DPPH free radical scavenging rate of the corresponding extract were 4.13% and 92.69% respectively, and it was an ideal solvent for industrial development and utilization of Camellia oleifera fruit hull to extract flavonoids. |
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