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| Preparation of soybean lysophospholipids and its biosafety analysis |
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| DOI： |
| KeyWord:soybean phospholipid phospholipase A1 soybean lysophospholipids hemolysis cytotoxicity |
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| Abstract: |
| Soybean lysophospholipids are modified phosphatides of losing a fatty acid group in the original structure of soybean phospholipids, which are more hydrophilic and antioxidative than soybean phospholipids, and are widely used in medicine, food and animal husbandry. Phospholipase A1 was used to modify the soybean phospholipids, and the degree of modification was detected by the hydrolysis rate and infrared spectrum, and the biological safety was detected by hemolytic test and cytotoxicity test. The results showed that the soybean lysophospholipids were prepared from the 40% soybean phospholipids in the water catalyzed by phospholipase A1 at 60?℃. The purified soybean lysophospholipids were block-brown material without obvious soybean smell. The hydrolysis rate of soybean lysophospholipids was 101.6%. The results of infrared spectra showed that the double bond and amide bond were significantly decreased in soybean lysophospholipids. Meanwhile, both of soybean phospholipids and soybean lysophospholipids had hemolytic phenomena, and their hemolysis rates at 200 μg/mL were less than 5%, which could be considered as biosafety. The cell survival rates were all higher than 80% when soybean phospholipids were below 50 μg/mL and soybean lysophospholipids were below 20 μg/mL. In summary, the prepared soybean lysophospholipids were biosafe at certain mass concentration. |
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