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| Microwave-assisted and enzyme-assisted aqueous extraction process of soybean oil and its mechanism |
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| KeyWord:microwave enzyme-assisted aqueous extraction process soybean oil extraction process protein structure |
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| Abstract: |
| The microwave-assisted and enzyme-assisted aqueous extraction process (EAEP) of soybean oil and its mechanism were studied. The soybean was pretreated by microwave, then was hydrolyzed by Protex 6L alkaline protease to extract oil. On the basis of single factor experiment, the process conditions were optimized by response surface methodology. The soybean treated by microwave was analyzed by transmission electron microscope, and the protein was analyzed by Raman spectroscopy. The results showed that the influence of microwave time on total oil extraction rate was the greatest, followed by Protex 6L dosage, ratio of liquid to material and microwave power. The optimal process conditions were obtained as follows: microwave power 700 W, microwave time 7.6 min, Protex 6L dosage 0.90% and ratio of liquid to material 5.15∶?1. Under these conditions, the total oil extraction rate was 92.13%. The soybean oil extracted by microwave-assisted and enzyme-assisted aqueous extraction process had better quality . The peroxide value, p-fennel value and phospholipid content were lower. The transmission electron microscopy showed that the cell wall structure tissue was destroyed more obviously after microwave treatment, which was an important reason for the increase of total oil extraction rate. The Raman spectroscopy showed that the protein was denatured after microwave treatment, which was also a reason for the increase of total oil extraction rate. |
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