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Optimization of enzymatic degumming of four grade fragrant rapeseed oil
  
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KeyWord:four grade fragrant rapeseed oil  enzymatic degumming  phospholipid content  acid value  yield
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WANG Zengqian，BAO Lilin，XIONG Weilin，etc  
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Abstract:
      The enzymatic degumming process of four grade fragrant rapeseed oil using phospholipase A1 was studied. Effects of enzyme dosage, 50% citric acid dosage, deionized water dosage and enzymolysis time on enzymatic degumming of four grade fragrant rapeseed oil were investigated.On the basis of single factor experiment, the enzymatic degumming conditions of four grade fragrant rapeseed oil were optimized by response surface methodology.The results showed that the optimal enzymatic degumming conditions were determined as follows: water bath temperature 55?℃, acid reaction time 3 min, enzyme dosage 22 mg/kg, 50% citric acid dosage 260 mg/kg, deionized water dosage 2% and enzymolysis time 120 min. Under these conditions, the phospholipid content of enzymatic degummed oil was 22.26 mg/kg, the acid value was 2.31 mgKOH/g, and the yield was 96.81%.
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