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四级浓香菜籽油酶法脱胶工艺条件优化
Optimization of enzymatic degumming of four grade fragrant rapeseed oil
  
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中文关键词:  四级浓香菜籽油  酶法脱胶  磷脂含量  酸价  收率
英文关键词:four grade fragrant rapeseed oil  enzymatic degumming  phospholipid content  acid value  yield
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Author NameAffiliation
WANG Zengqian，BAO Lilin，XIONG Weilin，etc  
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中文摘要:
      选取磷脂酶PLA1，对四级浓香菜籽油进行酶法脱胶工艺研究。分别研究了酶添加量、50%柠檬酸添加量、去离子水添加量、酶解时间对四级浓香菜籽油酶法脱胶效果的影响。在单因素实验的基础上，采用响应面实验设计，对酶法脱胶工艺条件进行优化。结果表明：四级浓香菜籽油酶法脱胶最佳工艺条件为水浴温度55?℃、酸反应时间3 min、酶添加量22 mg/kg、50%柠檬酸添加量260 mg/kg、去离子水添加量2%、酶解时间120 min，在该条件下酶法脱胶油磷脂含量为22.26 mg/kg，酸价（KOH）为 2.31 mg/g，收率为96.81%。
英文摘要:
      The enzymatic degumming process of four grade fragrant rapeseed oil using phospholipase A1 was studied. Effects of enzyme dosage, 50% citric acid dosage, deionized water dosage and enzymolysis time on enzymatic degumming of four grade fragrant rapeseed oil were investigated.On the basis of single factor experiment, the enzymatic degumming conditions of four grade fragrant rapeseed oil were optimized by response surface methodology.The results showed that the optimal enzymatic degumming conditions were determined as follows: water bath temperature 55?℃, acid reaction time 3 min, enzyme dosage 22 mg/kg, 50% citric acid dosage 260 mg/kg, deionized water dosage 2% and enzymolysis time 120 min. Under these conditions, the phospholipid content of enzymatic degummed oil was 22.26 mg/kg, the acid value was 2.31 mgKOH/g, and the yield was 96.81%.
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