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| Adulteration detection of Eucommia ulmoides seed oil using synchronous fluorescence spectroscopy |
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| KeyWord:Eucommia ulmoides seed oil fluorescence spectroscopy adulteration detection |
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| Abstract: |
| To establish an adulteration calibration model and detection method for Eucommia ulmoides seed oil by synchronous fluorescence spectroscopy, spectral characteristics of Eucommia ulmoides seed oil and seven vegetable oils ranged from 250-700 nm at the wavelength interval of 60 nm were analyzed, and the adulteration discriminant model was established using spectra peak area and calibrated. The results showed that there were significant differences in fluorescence spectral charateristics of Eucommia ulmoides seed oil and other seven vegetable oils. Principal component analysis of synchronous fluorescence spectroscopy of the oils ranged 300-500 nm and 600-700 nm were conducted, and the recognition accuracy rate of adulterated calibration of Eucommia ulmoides seed oil with other seven vegetable oils was 100%. The quantitative calibrations models based on fluorescence spectral peak area and adulteration ratio ranged 300-500 nm and 600-700 nm were established and the detection limits were 0.48% and 1% respectively. The synchronous fluorescence spectroscopy could be used for qualitative and quantitative analysis of adulteration of Eucommia ulmoides seed oil, and had the advantages of high sensitivity, simplicity and rapidness. |
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