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| 棉籽及棉籽相关产品中黄曲霉毒素B1含量的测定及迁移规律分析 |
| Determination and migration rule of aflatoxin B1 in cottonseed and its related products |
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| DOI： |
| 中文关键词: 黄曲霉毒素B1 棉籽及棉籽相关产品 免疫亲和柱层析 净化 高效液相色谱法 迁移规律 |
| 英文关键词:aflatoxin B1 cottonseed and its related products immunoaffinity chromatography cleanup high performance liquid chromatography migration rule |
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| 中文摘要: |
| 建立了棉籽及棉籽相关产品中黄曲霉毒素B1含量的测定方法。将样品混合均匀粉碎，称样量5 g，提取溶剂为86%乙腈溶液，固液比1∶?10，超声提取10 min，提取液经免疫亲和柱层析净化后，利用高效液相色谱法检测其中的黄曲霉毒素B1，并对黄曲霉毒素B1的迁移规律进行分析。结果表明，该方法的日内精密度为1.82%~4.41%，日间精密度为6.00%，样品加标回收率为97%~108%，可满足日常实际检测及质量控制要求。在棉籽加工过程中，黄曲霉毒素B1绝大部分迁移到棉籽蛋白中，棉籽油中几乎没有。这一结果说明生产过程中黄曲霉毒素B1污染主要来源于棉籽原料且易迁移到棉籽蛋白中。 |
| 英文摘要: |
| A method for determing aflatoxin B1 content in cottonseed and its related products was established.The sample was well mixed and crushed,then 5 g samples was solved in 86% acetonitrile aqueous solution with solid-liquid ratio 1∶?10,and extracted for 10 min with ultrasound assist,and the aflatoxins B1 in the extract were determined by HPLC after cleanup by immunoaffinity chromatography. The migration rule of aflatoxin B1 in cottonseed was also analyzed.The results showed that the intraday precision, daytime precision and recovery rate of standard addition of the method were 1.82%-4.41%,6.00% and 97%-108%,respectively,so the method could meet the daily actual testing and quality control requirements. In cottonseed processing, most of aflatoxin B1 migrated to cottonseed protein, and there was almost no in cottonseed oil. These results indicated that aflatoxin B1 in cottonseed products mainly came from contaminated cottonseed and easily migrated to cottonseed protein. |
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