Screening of high oil-producing Chlorella vulgaris by low-temperature plasma mutation
  
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KeyWord:low-temperature plasma  Chlorella vulgaris  mutation breeding  lipid content
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WEI Zhijin, LI Xiao, WANG Haonan,etc  
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Abstract:
      The Chlorella vulgaris was irradiated by low-temperature plasma, and the biomass, lipid content and fatty acid composition of the screened mutants in the incubation periods were analyzed so as to obtain the mutant with fast growth and high oil-producing. The results showed that three strains of microalgae with high lipid content were isolated. The total lipid content, neutral lipid content, total lipid productivity and neutral lipid productivity of the mutant M1 were 37.28%, 19.12%, 8.20 mg/(L·d) and 4.21 mg/(L·d), respectively, which increased by 103.5%, 133.5%, 163.7% and 202.9% compared with the wild strain. The biomass and protein content of M1 increased to some extent. Compared with the wild strain, the content of MUFA and PUFA in M1 algae oil increased by 23.6% and 329%, and their contents were 44.0% and 19.8%, respectively. Compared with the wild strain, C18∶?1 in M1 algae oil as a biodiesel index also increased by 64.9%.
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