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| 磁性松香基高分子固定化交联脂肪酶聚集体的制备及性质研究 |
| Preparation and property of immobilized cross-linked enzyme aggregates on magnetic rosin-based polymer |
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| DOI： |
| 中文关键词: 磁性松香基高分子 交联脂肪酶聚集体 脂肪酶 交联 固定化CLEAs-L |
| 英文关键词:magnetic rosin-based polymer cross-linked enzyme aggregates (CLEAs) lipase cross-linking immobilized CLEAs-L |
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| 中文摘要: |
| 制备了磁性松香基高分子固定化交联脂肪酶聚集体（固定化CLEAs-L）。研究制备条件对固定化CLEAs-L活性的影响，并研究了固定化CLEAs-L的结构与性质。结果表明，固定化CLEAs-L的最佳制备条件为：沉淀剂无水乙醇用量30%，脂肪酶质量浓度4 g/L，一次交联反应中添加0.3%的戊二醛，交联反应2 h，二次交联反应中添加1%的戊二醛，交联脂肪酶聚集体与载体的质量比2.5∶?1。在最佳条件下，固定化CLEAs-L的酶活回收率为86.52%，固定化CLEAs-L的最适温度和pH分别为45?℃和7.0。与游离脂肪酶、CLEAs-L相比，固定化CLEAs-L热稳定性和储存稳定性明显提高；重复操作6次后，固定化CLEAs-L的酶活回收率仍保持在60.00％以上。 |
| 英文摘要: |
| The immobilized cross-linked enzyme aggregates (CLEAs-L) on magnetic rosin-based polymer were prepared. The effects of preparation conditions on the activity of immobilized CLEAs-L were evaluated. Moreover, the properties and structures of immobilized CLEAs-L were investigated. The results showed that the optimal preparation conditions were obtained as follows: dosage of precipitant (absolute ethanol) 30%, lipase mass concentration 4 g/L, glutaraldehyde dosage 0.3% in the first cross-linking reaction, reaction time 2 h, glutaraldehyde dosage 1% in the second cross-linking reaction, and the mass ratio of CLEAs-L to carrier 2.5∶?1. Under these conditions, the recovery rate of enzyme activity was 86.52%, and the optimal temperature and pH of immobilized CLEAs-L were 45 ?℃ and 7.0 respectively. The thermal and storage stability of immobilized CLEAs-L were improved remarkably compared with the free lipase and CLEAs-L. After six batch reactions, the recovery rate of enzyme activity of immobilized CLEAs-L was still above 60.00%. |
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