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| Preparation of antioxidant peptides from whey protein and its in vitro antioxidant activity |
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| DOI： |
| KeyWord:whey protein antioxidant peptides enzymolysis in vitro antioxidant activity |
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| Abstract: |
| The antioxidant peptides were prepared by the hydrolysis of whey protein with proteases, and its in vitro antioxidant activity was discussed. The results showed that the neutral protease was the best enzyme for the protein hydrolysis with the hydroxyl free radical scavenging rate and peptides content as indicators. Response surface methodology was carried out based on single factor experiment. The optimal hydrolysis conditions were obtained as follows: pH 5.50, hydrolysis temperature 65?℃, hydrolysis time 1.65 h ，substrate mass fraction 5% and enzyme dosage 5 000 U/g. Under these conditions, the hydroxyl free radical scavenging rate of antioxidant peptides from whey protein was 74.54%.The antioxidant peptides from whey protein had good scavenging capabilities on hydroxyl free radical, ABTS+ free radical, DPPH free radical and superoxide anions free radical, and the IC50 values were 2.174, 0.709, 2.813 mg/mL and 4.579 mg/mL respectively, which indicated that antioxidant peptides from whey protein had strong in vitro antioxidant activity, and had certain development and utilization value. |
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