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Extraction of protein from peony seed cake by alkali extraction and acid precipitation method
  
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KeyWord:peony seed cake  peony seed protein  alkali extraction and acid precipitation  orthogonal experiment
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XU Yue, ZHANG Cunlao, YANG Gen，etc  
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Abstract:
      The peony seed cake was used as material, and the extraction conditions of protein with alkali extraction and acid precipitation were optimized using single factor experiment and orthogonal experiment. The optimal extraction conditions of protein from peony seed cake were obtained as follows：solid-liquid ratio 1∶?12, alkaline solution pH 8.5, extraction time 60 min, extraction temperature 45?℃. Under these conditions，the yield of peony seed protein was（78.23±0.04）%. The optimal pH of acid precipitation was 4.0, and the precipitation rate of protein was（90.90±0.11）%.
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