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| 酶法合成中长碳链甘油三酯 |
| Enzymatic synthesis of medium and long chain triacylglycerols |
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| DOI： |
| 中文关键词: 中长碳链甘油三酯 NS40086脂肪酶 酯交换 分子蒸馏 |
| 英文关键词:medium and long chain triacylglycerols NS40086 lipase interesterification molecular distillation |
| 基金项目: |
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| 中文摘要: |
| 以椰子油和富含1,3-二油酸-2-棕榈酸甘油三酯（OPO）和1-油酸-2-棕榈酸-3-亚油酸甘油三酯（OPL）的油脂为原料，NS40086脂肪酶为催化剂，酶法合成中长碳链甘油三酯（MLCT）。确定反应体系并探究底物摩尔比、反应温度、加酶量和反应时间对产物中MLCT含量的影响，并采用分子蒸馏纯化产物。结果表明：在底物摩尔比0.8∶?1、反应温度65?℃、加酶量8%、反应时间6 h、无溶剂体系条件下，产物中MLCT的含量为70.44%；分子蒸馏后产物中甘油三酯含量达97.22%；通过UPLC-Q-TOF-MS分析甘油三酯组成，结果表明酯交换反应生成了与人乳脂结构相似的MLCT，具有代表性的有OPLa、LPLa、OLaO、LLaO、OPCa、OMLa、LLLa、OLaLa、LLaLa等。 |
| 英文摘要: |
| Medium and long chain triacylglycerols (MLCT) were prepared by enzymatic interesterification of coconut oil and structured lipids rich in 1, 3-dioleoyl-2-palmitoylglycerol (OPO) and 1-oleoyl-2-palmitoyl-3-linoleoylglycerol (OPL). The reaction system was determined and the effects of substrate molar ratio, reaction temperature, enzyme dosage and reaction time on the content of MLCT in the product were investigated. The product was purified by molecular distillation. Under conditions of molar ratio of substrate 0.8∶?1, enzyme dosage 8%, reaction temperature 65?℃, reaction time 6 h and solven-free system the content of MLCT in the product was 70.44%. The content of triacylglycerols in the product reached 97.22% after molecular distillation. The species of triacylglycerols were identified by UPLC-Q-TOF-MS, and the results showed that the MLCT in the product were similar to that in human milk fat, including OPLa,LPLa,OLaO, LLaO, OPCa, OMLa, LLLa, OLaLa, LLaLa, etc. |
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