| In order to improve the biomass and lipid productivity of Haematococcus pluvialis, the H. pluvialis with the concentration of 1×107 cells/mL and Paecilomyces lilacinus TD16 with the concentration of 1.36×107 cells/mL were mixed and cultured in different proportions, and the optimal inoculation volume ratio was 5∶?1. Compared with individual culture, H. pluvialis in mixed culture：the biomass (0.92 g/L) and average growth rate (62.30 mg/(L·d)) increased by 24.32% and 31.66% respectively, while doubling time shortened from 4.26 d to 3.24 d; lipid productivity (184.91 mg/L) and the average lipid synthesis rate (13.21 mg/(L·d)) increased by 45.59% and 45.64% respectively, C16∶?0 and C18∶?1 contents increased by 20.56% and 19.27%, respectively; the highest mass concentrations of chlorophyll a (3.89 mg/L) and astaxanthin (4.37 mg/L) increased by 20.81% and 35.71% respectively. These results demonstrated that the mixed culture of P. lilacinus TD16 and H. pluvialis could promote the cell growth and lipid synthesis. |
