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| 响应面法优化球磨破碎圆红冬孢酵母细胞工艺条件 |
| Optimization of R. toruloides cell disruption technology by ball milling method using response surface methodology |
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| DOI： |
| 中文关键词: 球磨法 圆红冬孢酵母 细胞破碎 响应面法 工艺优化 |
| 英文关键词:ball milling method R. toruloides cell disruption response surface methodology process optimization |
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| 中文摘要: |
| 为探究工业条件成熟的物理球磨法对圆红冬孢酵母细胞的破碎效果，采用油脂得率为评价指标，考察了研磨介质、研磨时间、球磨机转速、介质添加量4个关键性因素的影响。在单因素试验的基础上，利用响应面法确定了最佳的细胞破碎工艺条件。结果表明：最佳细胞破碎工艺条件为研磨介质为硅酸锆珠、研磨时间2.5 h、球磨机转速360 r/min、介质添加量（以酵母液体积计）41%，在此工艺条件下油脂得率为57.32%。 |
| 英文摘要: |
| In order to explore the disruption effect of physical ball milling method with mature industrial conditions on R. toruloides cell, with oil yield as the evaluation index, the effects of four critical factors including milling media, milling time, ball mill speed and dosage of media were studied. On the basis of single factor experiment, the optimal cell disruption process conditions were obtained by response surface methodology as follows： zirconium silicate bead used as milling media, milling time 2.5 h, ball mill speed 360 r/min and dosage of media (based on yeast liquor volume ) 41%. Under these conditions, the oil yield was 57.32%. |
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