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| Steam explosion-assisted extraction of oil-tea camellia seed protein and its functional properties |
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| KeyWord:steam explosion oil-tea camellia seed protein alkali extraction and acid precipitation functional property |
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| Abstract: |
| The steam explosion-assisted alkali extraction and acid precipitation method was used to extract oil-tea camellia seed protein, and the optimal extraction conditions were determined. The effects of pH, protein mass concentration, NaCl and sucrose concentrations on the functional properties of oil-tea camellia seed proteins before and after steam explosion were analyzed. The results showed that the optimal extraction conditions were obtained as follows: steam explosion pressure 0.8-2.3 MPa, steam explosion time 30-120 s,solid-liquid ratio 1∶?10, extraction temperature 40?℃, pH 10 and extraction time 50 min. Under these conditions, the extraction rate of oil-tea camellia seed protein was 71.01%,significantly higher than that of untreated oil-tea camellia seed protein(58.74%), and the oil absorption and water absorption were improved. The foaming properties and emulsifying properties of oil-tea camellia seed protein were affected by pH, protein mass concentration, NaCl concentration. And the sucrose concentration had a significant effect on the foaming ability of the protein, but had no significant improvement on the foam stability, and it improved the emulsifying ability and emulsion stability of the protein. |
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