|
| Pretreatment of purification and enrichment for determination of bitter peptides in flaxseed oil by HPLC |
| |
| DOI： |
| KeyWord:flaxseed oil bitter peptides pretreatment purification and enrichment solid phase extraction method |
| FundProject: |
|
| Hits: 1527 |
| Download times: 0 |
| Abstract: |
| The purification and enrichment effects of solvent extraction method and solid phase extraction(SPE) method on the quantitative determination of bitter peptide(CLE) in flaxseed oil by HPLC were compared. Furthermore, the pretreatment effects of SPE silica gel column with different filler specifications were compared. The results showed that the flaxseed oil pretreated by the solvent extraction method and the SPE method was quantitatively determined by HPLC, and the CLE could be well separated, and the pretreatment methods did not affect the peak time and peak shape of the CLE. However, compared with the solvent extraction method, triglyceride and other impurities in cyclolinopeptide sample extracted by the SPE method significantly decreased, which was extremely advantageous for protecting the chromatography column and improving the column efficiency. With the increase of the filler amount of SPE silica gel column, the CLE measurement value decreased. A good separation and enrichment effect could be obtained by using a SPE silica gel column with a filler amount of 0.5 g. The results of UPLC-MS analysis of SPE silica gel column pretreatment showed that it was indeed CLE. The SPE method could effectively extract CLE from flaxseed oil, and it was suitable for pretreatment of HPLC quantitative determination of CLE. The recovery of standard addition of the method was 92.62%, and the RSDs of the precision within the group and between the groups were 0.62% and 1.79% respectively. |
| View full text View/Add Comment Download reader |
| Close |
|
|
|
