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Optimization of ethanol extraction of fish roe oil from large yellow croaker by response surface methodology |
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DOI: |
KeyWord:large yellow croaker fish roe oil extraction fatty acid |
FundProject:国家自然科学基金(318014631701710);福建省自然科学基金(2017J05047) |
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Abstract: |
In order to improve the comprehensive utilization of large yellow croaker roe, the ethanol extraction conditions of fish roe oil from large yellow croaker were optimized, and the fatty acid composition of fish roe oil was analyzed. The effects of ratio of material to liquid, extraction temperature and extraction time on the yield of fish roe oil were studied by single factor experiment. Then the extraction conditions were optimized by response surface methodology based on Box-Behnken central composite design. The results showed that the optimal extraction conditions of fish roe oil from large yellow croaker were obtained as follows:ratio of material to liquid 1∶ 14, extraction temperature 55 ℃ and extraction time 5.8 h. Under these conditions, the average yield of fish roe oil was (30.23±0.03)%. The fish roe oil from large yellow croaker was rich in polyunsaturated fatty acids, and the total content of DHA and EPA were (21.9±0.83)%. |
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