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| Preparation of flaxseed protein concentrate by ethanol extraction |
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| DOI： |
| KeyWord:flaxseed protein concentrate protein content ethanol extraction |
| FundProject:国家自然科学基金(31101367)；国家麻类产业技术体系信阳麻类综合试验站资金资助（CARS-16S09） |
| Author Name | Affiliation | | ZHANG Lixia1, ZHANG Zhenshan2, SHI Pengfei1 , GUO Xiaoyan1 , LING Jingwei1 ,
CHEN Xueqing1, L Yuhu1 , NIE Liangpeng1 , ZHANG Lin1 , PAN Ziliang1 | 1.Xinyang City Academy of Agricultural Sciences，Xinyang 464000, Henan,China
2.College of Food
Science and Technology, Henan University of Technology, Zhengzhou 450001, China |
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| Abstract: |
| Cold-pressed flaxseed cake was used as raw material,flaxseed protein concentrate was prepared by ethanol extraction. The effects of extraction temperature, extraction time, ethanol volume fraction, liquid-solid ratio and extraction times on protein content in the prepared flaxseed protein concentrate were studied by single factor experiment, then orthogonal experiment was used to optimize the parameters. The results showed that the importance order of each factor from high to low was ethanol volume fraction, extraction time, extraction temperature, liquid-solid ratio, and the optimal parameters were obtained as follows: extraction temperature 50 ℃, extraction time 75 min, ethanol volume fraction 70%,liquid-solid ratio 6∶ 1 and extraction times twice. Under the optimal conditions, the protein recovery and content of flaxseed protein concentrate were 63.87% and 65.38%, respectively. |
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