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| Preparation of high purity stigmasterol with ultrasound-assisted fractional crystallization |
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| DOI： |
| KeyWord:phytosterol stigmasterol ultrasound-assist enrichment purification |
| FundProject:植物甾醇和植物甾醇酯的行业标准研究（18DZ2200300）；上海市2018年度“科技创新行动计划”技术标准项目 |
| Author Name | Affiliation | | FENG Wenhuan1, WU Zhengzhang2, ZHANG Peng2, CHANG Ming1,
JIN Qingzhe1, WANG Xingguo1 | 1.National Functional Food Engineering Technology Research Center, School of Food
Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu,China
2.Jiangsu CONAT Biological Products Co., Ltd., Taixing 225400,Jiangsu,China |
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| Abstract: |
| Stigmasterol was separated and purified from phytosterol by two-step method of enrichment and purification. Stigmasterol was firstly enriched by crystallization with non-polar solvent，then purified by ultrasound-assisted recrystallization with polar solvent. The conditions of enrichment and purification processes of stigmasterol were optimized by single factor experiment. The results showed that the purity of stigmasterol reached 94.69% by five times crystallization under the optimal enrichment conditions of with cyclohexanone as solvent,ratio of material to liquid 1∶ 3.5，crystal growth temperature 25 ℃ and crystal growth time 16 h. And the purity of stigmasterol increased to 99.36% by twice recrystallization under the optimal purification conditions of with acetone as solvent,ultrasonic time 3 min,ultrasonic power 90 W,ratio of material to liquid 1∶ 70，crystal growth temperature 25 ℃ and crystal growth time 3 h. The samples were identified as stigmasterol by MS，FT-IR and NMR. |
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