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| Stability and gastrointestinal biomimetic digesting patterns of peanut peptide-ferrous |
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| DOI：10.12166/j.zgyz.1003-7969/2019.11.007 |
| KeyWord:：peanut peptide-ferrous coordinating chelation gastrointestinal biomimetic digesting biomimetic digesting pattern peanut peptide |
| FundProject:湖南省自然科学基金科教联合基金项目（2018JJ5022）；湖南省教育厅科研项目（18C1356）；湖南化工职业技术学院校级科研项目（HNHY2017013） |
| Author Name | Affiliation | | XIAO Huaiqiu1,2 , LI Yuzhen1,LIN Qinlu3 , ZHAO Mouming2 ,
LIU Jun1 ,ZHOU Quan1 ,JIANG Mingjiao4 | 1.School of Pharmaceutical and Bioengineering, Hunan Chemical Vocational Technology College, Zhuzhou 412000,
Hunan,China
2.School of Food Science and Engineering, South China University of Technology,
Guangzhou 510640, China
3.College of Food Science and Technology, Central South
University of Forestry and Technology, Changsha 410004, China
4.Hunan Zonwe
Pharmaceutical Co., Ltd., Zhuzhou 412000,Hunan,China |
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| Abstract: |
| In order to study the gastrointestinal biomimetic digestion patterns and degradation law in human gastrointestinal tract after oral administration of peanut peptide-ferrous chelator, L-PPC, M-PPC and H-PPC were synthesized by adopting peanut peptides with different relative molecular weight (<1.0 kDa, 1.0-3.0 kDa, >3.0 kDa) as carrier and ferrous chloride as ligand, and their stability and gastrointestinal digestion tolerance were investigated. The results indicated that the pH stability of L-PPC was better than that of M-PPC and H-PPC with a significant difference (p<0.01), and the pH stability of H-PPC was the worst; H-PPC had the worst heat tolerance, especially when the temperature was over 50 ℃, the ferrous chelating rate was less than 50%, which was significantly different from L-PPC and M-PPC(p<0.01); L-PPC had the best thermal tolerance. The gastric acid tolerance of L-PPC was significantly higher than that of M-PPC and H-PPC. The difference for ferrous chelating rate between L-PPC and M-PPC was not significant (p>0.05), but significantly different from H-PPC (p<0.01) for 30 min of gastric biomimetic digestion. The ferrous chelating rate of M-PPC and H-PPC were (71.83%±1.32)%, (56.61%±1.16)% and (61.46%±125)%, (53.90%±133)% for 90 min and 120 min of gastric biomimetic digestion, respectively. After gastric biomimetic digestion for 120 min, the residual chelating rates of L-PPC, M-PPC and H-PPC were 91.15%, 69.05% and 74.10% respectively, compared with the sample of gastric biomimetic digestion for 30 min. After biomimetic digestion for 60 min in duodenum, the ferrous chelating rate of M-PPC and H-PPC decreased to (57.93±0.83)% and (45.65±0.87)%, and after digestion for 180 min in small intestine, their ferrous chelating rates were (38.42± 0.85)%,(18.34±0.72%), respectively, which were significantly different from L-PPC (p<0.01). The L-PPC had the better intestinal tolerance and H-PPC was the worst. All results showed that L-PPC possesses the better pH stability, heat tolerance and gastrointestinal digestion tolerance compared with M-PPC and H-PPC. |
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