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| Determination of aflatoxins in edible oils by ultrasound-assisted-dispersive liquid-liquid microextraction(UA-DLLME) /RRLC-MS/MS |
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| DOI：10.12166/j.zgyz.1003-7969/2019.11.019 |
| KeyWord:ultrasound-assisted-dispersive liquid-liquid microextraction RRLC-MS/MS aflatoxin edible oil |
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| Abstract: |
| A method of ultrasound-assisted-dispersive liquid-liquid microextraction(UA-DLLME) and rapid resolution liquid chromatography-tandem mass spectrometry （RRLC-MS/MS）was estabished to detect aflatoxins(AFB1, AFB2, AFG1, AFG2) in edible oils. The sample was dissolved in dispersing agent, extracted by extractant, then the bottom solution after centrifugation was defatted and injected into RRLC-MS/MS. Target aflatoxins were analyzed in positive electrospray ionization(ESI+) under multiple reaction monitoring(MRM) mode. The sample pretreatment method was optimized. The results showed that the optimal pretreatment conditions were obtained as follows: 1 g sample, 2 mL petroleum ether as dispersing agent, 1 000 μL acetonitrile-water(volume ratio 8∶ 2) as extractant, UA extraction time 2 min, centrifugation time 5 min, centrifugation speed 4 000 r/min. The linearity of the established method was in the range of 0.625-30.0 ng/mL, the correlation coefficients were above 0.998, the recoveries of spiked samples were in the range of 88.2%-101.5% and the relative standard deviations were less than 3.3%.The method had items of simpleness, rapidness, low cost, high accuracy and precision, environmental friendliness and was suitable for qualitative and quantitative analysis of aflatoxins in edible oils. |
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