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| 栀子油对对乙酰氨基酚致小鼠急性肝损伤的保护作用 |
| Protective effect of Gardenia oil on acetaminophen-induced acuteliver injury in mice |
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| DOI：10.12166/j.zgyz.1003-7969/2019.11.026 |
| 中文关键词: 栀子油 对乙酰氨基酚 脂质过氧化 急性肝损伤 |
| 英文关键词:Gardenia oil acetaminophen lipid peroxidation acute liver injury |
| 基金项目:国家重点研发计划中医药现代化研究重点专项（2017YFC1700902）；国家公益性科研专项（201507002） |
| Author Name | Affiliation | | ZHANG Junyi1, LUO Guangming1,2, CHAI Huawen1, LIU Peipei1, ZHU Zihao1 | 1.School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004,China
2.Research Center of Chinese Herbal Medicine and Ethnic Medicine, Jiangxi University of
Traditional Chinese Medicine, Nanchang 330004,China |
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| 中文摘要: |
| 探讨栀子油对对乙酰氨基酚致小鼠急性肝损伤的保护作用及机制。将实验小鼠随机分成正常对照组，模型组，联苯双酯组，栀子油低、中、高剂量组，各给药组每日给药1次，连续7 d，末次给药1 h后，除正常对照组外，其他各组一次性腹腔注射对乙酰氨基酚（450 mg/kg），测定小鼠血清中ALT、AST和ALB水平及肝脏组织匀浆中GSH、SOD活力和MDA含量，HE染色观察肝组织的病理学变化。结果表明：与模型组相比，栀子油各剂量组均能降低血清ALT和AST水平，提高ALB水平及GSH、SOD活力，降低肝组织MDA的产生，肝组织变性及坏死等病理症状明显改善，尤以栀子油高剂量组效果最佳。栀子油对对乙酰氨基酚诱导小鼠急性肝损伤有明显的保护作用，其保肝的机制可能与提高抗氧化酶体系活力、抑制脂质过氧化、维持肝细胞膜正常形态有关。 |
| 英文摘要: |
| The protective effect of Gardenia oil on acetaminophen(AP)-induced acute liver injury in mice and mechanism were investigated. The mice were randomly divided into control group, model group, biphenyl dimethylester group, and low, middle and high dose groups of Gardenia oil, and administrated once a day for 7 d,and 1 h after the last administration, the mice were intraperitoneally injected with AP of 450 mg/kg in each group except for the control group．The contents of ALT, AST and ALB in the blood serum, the activity of GSH and SOD, and the level of MDA in the liver organs of mice were determined, and pathological morphological changes in liver cells were monitored by HE staining. The results showed that compared with model group, each dose group of Gardenia oil could reduce the contents of ALT and AST, improve the content of ALB and the activities of GSH and SOD, and reduce the production of MDA in liver tissue, pathological damage in liver such as liver tissue degeneration and necrosis were significantly improved, especially in the high dose group of Gardenia oil. The results suggested that Gardenia oil had markedly protective effect on AP-induced acute liver injury in mice, and the mechanism may be related to its ability of improving the activity of antioxidant enzyme system, inhibiting lipid peroxidation and maintaining normal morphology of liver cell membrane. |
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