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| Enrichment of linoleic acid from Pinus armandi franch seed oil and its lipid-lowering activity |
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| DOI：10.12166/j.zgyz.1003-7969/2019.11.027 |
| KeyWord:Pinus armandi franch seed oil linoleic acid urea adduction fractionation enrichment lipid-lowering activity |
| FundProject:国家高技术研究发展计划“863计划”资助项目（2007AA100404）；天津市高等学校科技发展基金计划项目（20110614）；国家自然科学基金（81502963） |
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| Abstract: |
| Pinus armandi franch seed oil was saponified and acidified to obtain the mixed fatty acid，and then the linoleic acid was enriched by urea adduction fractionation.The effects of adduction temperature，adduction time, mass ratio value of mixed fatty acid to urea, ratio value of 95% ethanol volume to mixed fatty acid mass on purity and yield of linoleic acid were studied.Different dosage of linoleic acid obtained was administered to the hyperlipidemic mice to evaluate its lipid-lowering function.The optimal enrichment conditions of linoleic acid were determined by single factor experiment as follows：adduction temperature -10 ℃, adduction time 24 h, mass ratio value of mixed fatty acid to urea 0.4, ratio value of 95% ethanol volume to urea mass 5.Under these conditions, the purity of linoleic acid was 80.57% after once urea adduction. The results of lipid-lowering evaluation showed that the linoleic acid from Pinus armandi franch seed oil could reduce the body weight, liver weight, liver index, serum total cholesterol(TC) and triglyceride(TG) levels of the hyperlipidemic mice. It could improve the high density lipoprotein cholesterol(HDL-C) level, reduce the low density lipoprotein cholesterol(LDL-C) level and arteriosclerosis index(AI) in mice. The linoleic acid obtained from Pinus armandi franch seed oil could reduce the blood lipid level of hyperlipidemic mice and improve the accumulation of liver fat and atherosclerosis caused by excessive intake of lipids. |
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