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| Antibacterial activity and mechanism of emu oil |
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| DOI： |
| KeyWord:emu oil antibacterial activity antimicrobial mechanism |
| FundProject:山东省泰山产业领军人才创新类项目（LJNY2015007）；国家自然科学基金（31671786） |
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| Abstract: |
| Emu oil (EO) is widely recognized as a kind of natural antibacterial agent, however, the antibacterial mechanism has not been described yet. The effect of EO on the two typical food pathogens (Escherichia coli and Staphylococcus aureus) and their cellular membrane and intracellular substances were investigated. The results showed that EO could effectively inhibit the growth of Escherichia coli and Staphylococcus aureus, and the minimum inhibitory concentration were 0.006 25% and 0.012 5%, respectively. The influences of EO on the surface potential and hydrophobicity, activity of ATPase, β-galactosidase and alkaline phosphatase(AKPase) of this two pathogens were also detailedly studied. It further indicated that EO could reduce the surface potential, while improve the surface hydrophobicity, which was the main reasons of the antibacterial function. Besides, EO could reduce the cellular ATPase activity, thus reduce the metabolic rate. Moreover, EO showed no effect on the protein and DNA synthesis abilities,β-galactosidase and AKPase activities of Escherichia coli, while it could reduce the ability of macromolecular substances synthesis, the β-galactosidase and AKPase activities of Staphylococcus aureus. |
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