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| 鸸鹋油的抑菌活性及机理研究 |
| Antibacterial activity and mechanism of emu oil |
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| DOI： |
| 中文关键词: 鸸鹋油 抑菌活性 抑菌机理 |
| 英文关键词:emu oil antibacterial activity antimicrobial mechanism |
| 基金项目:山东省泰山产业领军人才创新类项目（LJNY2015007）；国家自然科学基金（31671786） |
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| 中文摘要: |
| 鸸鹋油被公认为具有抗菌性，但其抗菌机理尚不明确。通过探究鸸鹋油对食物中常见致病菌株（大肠埃希菌和金黄色葡萄球菌）的影响，分析其对供试菌细胞膜和胞内物质的作用。结果表明：鸸鹋油对大肠埃希菌和金黄色葡萄球菌生长均具有较好的抑制作用，对大肠埃希菌最小抑菌浓度为0.006 25%，对金黄色葡萄球菌最小抑菌浓度为0.012 5%。通过测定鸸鹋油对大肠埃希菌和金黄色葡萄球菌表面电位和疏水性，以及对其中ATP酶、 β-半乳糖苷酶和碱性磷酸酶的活性等的影响，进一步发现，鸸鹋油主要通过影响供试菌表面特性来抑制其生长，可以有效减弱细菌表面电荷负荷性，提高细菌表面疏水性，还可通过影响ATP酶活性改变其新陈代谢速率。鸸鹋油基本不影响大肠埃希菌蛋白和DNA合成能力、β-半乳糖苷酶以及碱性磷酸酶活性，但其能够降低金黄色葡萄球菌的相应大分子合成能力、β-半乳糖苷酶和碱性磷酸酶活性。 |
| 英文摘要: |
| Emu oil (EO) is widely recognized as a kind of natural antibacterial agent, however, the antibacterial mechanism has not been described yet. The effect of EO on the two typical food pathogens (Escherichia coli and Staphylococcus aureus) and their cellular membrane and intracellular substances were investigated. The results showed that EO could effectively inhibit the growth of Escherichia coli and Staphylococcus aureus, and the minimum inhibitory concentration were 0.006 25% and 0.012 5%, respectively. The influences of EO on the surface potential and hydrophobicity, activity of ATPase, β-galactosidase and alkaline phosphatase(AKPase) of this two pathogens were also detailedly studied. It further indicated that EO could reduce the surface potential, while improve the surface hydrophobicity, which was the main reasons of the antibacterial function. Besides, EO could reduce the cellular ATPase activity, thus reduce the metabolic rate. Moreover, EO showed no effect on the protein and DNA synthesis abilities,β-galactosidase and AKPase activities of Escherichia coli, while it could reduce the ability of macromolecular substances synthesis, the β-galactosidase and AKPase activities of Staphylococcus aureus. |
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