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Synthesis of human milk fat substitute rich in OPO by enzymatic acidolysis
  
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KeyWord:1,3-dioleoyl-2-palmitoylglycerol(OPO)  palm stearin  acidolysis  human milk fat substitute
FundProject:国家自然科学基金资助项目（31701559）
Author NameAffiliation
ZOU Shuo， WANG Xiaohan， WANG Xiaosan， JIN Qingzhe， WANG Xingguo Synergetic Innovation Center of Food Safety and Quality Control in Jiangsu Province， School of Food Science and Technology，Jiangnan University，Wuxi 214122，Jiangsu，China 
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Abstract:
      ：Using palm stearin and oleic acid as raw materials, human milk fat substitute rich in OPO was prepared by enzymatic acidolysis in solvent-free system with sn-1, 3-position specific lipase as catalyst. The reaction conditions were optimized by single factor experiment and the compositions of triglyceride and fatty acid in sn-2 position of the substitute were analyzed by high performance liquid chromatography and gas chromatography. The results showed that the optimal conditions of enzymatic acidolysis reaction were obtained as follows: reaction time 4 h, molar ratio of substrate 1∶ 10, reaction temperature 60 ℃ and dosage of lipase NS40086 12%. Under these conditions, the contents of OPO and palmitic acid in sn-2 position reached 72.53% and 87.03%, respectively.
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