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| 紫外辐射对小球藻光合性能及油脂积累的影响 |
| Effect of UV radiation on photosynthetic performance and lipid accumulation of Chlorella vulgaris |
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| DOI： |
| 中文关键词: 小球藻 紫外辐射 光合性能 油脂积累 荧光定量PCR 脂肪酸组成 |
| 英文关键词:Chlorella vulgaris UV radiation photosynthetic performance lipid accumulation fluorescent quantitative PCR fatty acid composition |
| 基金项目:“十三五”国家重点研发计划（2016YFC0701001-02）；陕西省科技统筹创新工程计划项目（2015KTCL-03-15） |
| Author Name | Affiliation | | SUN Xiaoqin1, SUN Xin1, LI Pengfei1, LIU Xingshe1,
CHENG Zhiwen2, LI Qing2 | 1.Key Laboratory of Northwest Water Resources, Environment and Ecology, Ministry of Education,
Key Laboratory of Environmental Engineering, Shaanxi Province, Xian University of Architecture and
Technology, Xian 710055, China
2.Xianyang Research & Design Institute of Ceramics,
Xianyang 712000, Shaanxi, China |
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| 中文摘要: |
| 在紫外辐射剂量0～80 mJ/cm2的条件下，探究紫外辐射对小球藻生长、光合性能和油脂积累的影响，利用荧光定量PCR测定藻细胞内与油脂合成相关基因的表达情况，同时采用气相色谱质谱联用测定小球藻油的脂肪酸组成及含量。结果表明：在最佳辐射剂量40 mJ/cm2条件下，小球藻的生物量达到515 mg/L，光合性能维持在较高水平，Fv/Fm、Y（Ⅱ）、油脂含量、油脂产量分别达到0.588、0594、46%、237 mg/L。相对于对照组，40 mJ/cm2剂量的小球藻的油脂含量、油脂产量分别提高了39.40%和39.22%，accD和gapA的相对表达量分别提高了约2.5倍和5倍，Me、Pk相对表达量基本不变。40 mJ/cm2剂量的小球藻油中单不饱和脂肪酸含量较高，达到40.17%，比对照组提高了16.5%,有利于生物柴油的生产，实际应用价值较高。 |
| 英文摘要: |
| The effects of UV radiation on the growth, photosynthetic performance and lipid accumulation of Chlorella vulgaris were studied under the radiation dose of 0-80 mJ/cm2. The expression of genes related to lipid synthesis in algae cells was determined by fluorescent quantitative PCR, and the compositions and contents of fatty acids in Chlorella vulgaris oil were determined by GC-MS. The results showed that under the optimal radiation dose of 40 mJ/cm2, the biomass of Chlorella vulgaris was up to 515 mg/L, and the photosynthetic performance was maintained at a high level, Fv/Fm, Y(II), lipid content and lipid productivity reached 0.588, 0.594, 46% and 237 mg/L, respectively. Compared with the control, the lipid content and productivity increased by 39.40% and 39.22%, respectively. The relative expressions of accD and gapA genes were 2.5 times and 5 times higher than those of control respectively, and the relative expressions of Me and Pk genes changed little. Compared with the control, the content of monounsaturated fatty acids in the Chlorella vulgaris oil improved by 165% and reached 40.17%, which was cond ucive to the production of biodiesel and had higher practical application value. |
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