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| 裂殖壶菌诱变株高产DHA机理的转录组学分析 |
| Transcriptome analysis for the mechanism of high-yield of DHA by mutagenic Schizochytrium |
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| DOI： |
| 中文关键词: 裂殖壶菌 DHA 转录组学分析 荧光定量PCR |
| 英文关键词:Schizochytrium DHA transcriptome analysis fluorescence quantitative PCR |
| 基金项目:江苏省高等学校自然科学研究面上项目（18KJB510045）；江苏省物联网应用技术重点建设实验室开放课题（18WXWL02） |
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| 中文摘要: |
| 利用RNA-seq技术对裂殖壶菌SR21的原始菌株和ARTP诱变所获取的DHA高产菌株NA2进行转录组分析。对基因进行功能注释和代谢通路的分类，寻找差异表达基因，并将这些基因归类到相关的代谢途径中，揭示诱变菌NA2中DHA产量提高的分子机理。结果表明：与原始菌株比较，诱变菌NA2中有314个基因上调，3个基因下调；上调的基因主要参与氨基酸代谢和能量代谢，为多不饱和脂肪酸的积累供应更多的乙酰辅酶A(乙酰-CoA)和还原型辅酶Ⅱ(NADPH)。利用荧光定量PCR验证基因的差异表达情况，结果与转录组分析一致。 |
| 英文摘要: |
| Transcriptome analysis for the original Schizochytrium limacinum SR21 strain and ARTP mutagenic strain NA2 with high yield of DHA was implemented with RNA-seq. The function of genes was annotated and metabolic pathways was classified to look for different expression genes and classify these genes into related metabolic pathways to reveal the mechanism of high yield of DHA in NA2. The results showed that 314 genes were up-regulated and three genes were down-regulated compared with the original strain. The up-regulated genes were mainly involved in amino acid metabolism and energy metabolism, which provided more acetyl-CoA and NADPH for the accumulation of polyunsaturated fatty acids. Validating the differently expressed genes with realtime fluorescence quantitative PCR, the result was in accordance with the transcriptome analysis. |
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