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| Ultrasound-assisted pretreatment for determination of secoisolariciresinol diglucoside in flaxseed by HPLC |
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| DOI： |
| KeyWord:flaxseed secoisolariciresinol diglucoside ultrasound pretreatment determination |
| FundProject:“十三五”国家重点研发计划（2018YFD0401103） |
| Author Name | Affiliation | | YU Congcong, HUANG Jianhua, LIU Ruijie, CHANG Ming, WANG Xiaosan,
WANG Xingguo, JIN Qingzhe | School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China |
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| Abstract: |
| Using flaxseed as raw material,the ultrasound-assisted pretreatment method for the determination of secoisolariciresinol diglucoside (SDG)in flaxseed by HPLC was studied. The effects of extraction solvent, ratio of material to liquid, ultrasonic time, ultrasonic temperature, ultrasonic power and extraction times on the content of SDG in flaxseed were investigated by single factor experiment. The results showed that the optimal ultrasound-assisted pretreatment conditions for the determination of SDG in flaxseed by HPLC were obtained as follows: with methanol as extraction solvent, ratio of material to liquid 1∶ 20, ultrasonic time 2 h, ultrasonic temperature 35 ℃, ultrasonic power 800 W and extraction times three times. Under the optimal conditions, the content of SDG in flaxseed was 11.40 mg/g. |
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