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| 测定菜籽油中酚类化合物样品前处理方法的比较 |
| Comparison of different pretreatment methods for the determination of phenolic compounds in rapeseed oil |
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| DOI： |
| 中文关键词: 菜籽油 酚类化合物 LLE SPE QuEChERS LC-MS/MS |
| 英文关键词:rapeseed oil phenolic compound LLE SPE QuEChERS LC-MS/MS |
| 基金项目:山东省泰山产业领军人才创新类项目(LJNY2015 007)；国家自然科学基金（31671786)；国家重点研发计划资助项目(2016YFD0401404) |
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| 中文摘要: |
| 采用液液萃取（LLE）、固相萃取（SPE）、QuEChERS 3种不同前处理方法提取菜籽油中酚类化合物，并采用超高效液相色谱串联质谱（LC-MS/MS）进行测定，分析比较了3种不同前处理方法对菜籽油中酚类化合物提取效果的影响。结果表明：在3种不同的前处理方法中，QuEChERS前处理方法的提取效果最好，对菜籽油中7种酚类化合物的回收率最高，可达85.16%~101.78%，基质共提物含量最低，为2.04 mg/g；LLE的回收率为66.04%~101.11%，基质共提物含量最高，为6.80 mg/g；SPE的回收率最低，仅为56.98%~83.79%，基质共提物含量为2.30 mg/g。 |
| 英文摘要: |
| The phenolic compounds in rapeseed oil were respectively extracted by liquid-liquid extraction (LLE), solid phase extraction (SPE) and QuEChERS, then determined by ultra performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). The effects of the three different pretreatment methods on the extraction efficiency of phenolic compounds in rapeseed oil were analyzed and compared. The results showed that QuEChERS method provided the best extraction efficiency among the three methods and the highest recovery of seven phenolic compounds, reaching 85.16%-101.78%. The content of matrix co-extract with QuEChERS method was the lowest, reaching 2.04 mg/g. The recovery of LLE method was 66.04%-101.11%, and the content of matrix co-extract was the highest, reaching 6.80 mg/g. The recovery of SPE method was the lowest, only 56.98%-83.79%, and the content of matrix co-extract was 2.30 mg/g. |
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