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| Influencing factors of demulsification with sesame endogenous protease |
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| DOI：10.12166/j.zgyz.1003-7969/2020.10.008 |
| KeyWord:sesame crude oil body endogenous protease demulsification |
| FundProject:国家自然科学青年基金（31301496） |
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| Abstract: |
| The hydrolysis properties of sesame endogenous protease were studied by Tricine-SDS-PAGE and SEC-HPLC. Under the optimal hydrolysis conditions of endogenous protease,the effects of hydrolysis time, demulsification pH, solid content, particle size and protein content on demulsification were investigated.The results showed that the hydrolysis rate of endogenous protease in crude sesame oil body was relatively the fastest at pH 4 and 50 ℃. And under these conditions hydrolysis for 4 h,the demulsification rate was 97% at demulsification pH 5. However, comprehensively considering the growth of demulsification rate and flavor, 2 h was the best choice and at this time the demulsification rate was 87%.When the solid content in crude oil body was 50%, the demulsification rate was the highest. The smaller the particle size,the higher the protein content,the more difficult it is to demulsify. These results provided technological basis for aqueous enzymatic extraction of oil by sesame endogenous protease. |
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