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| Preparation of fatty acid methyl ester by enzymatic transesterification using rapeseed oil deodorized distillate |
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| DOI：10.12166/j.zgyz.1003-7969/2020.10.016 |
| KeyWord:rapeseed oil deodorized distillate lipase transesterification fatty acid methyl ester |
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| Abstract: |
| The rapeseed oil deodorized distillate was esterified with methanol by chemical methods, and then was transesterified with methanol by enzymatic method to prepare fatty acid methyl ester. Under the condition of the Lipase EC 3.1.1.3 dosage 4%, the effects of methanol dosage, system pH, reaction temperature and reaction time on the transesterification conversion rate were studied by single factor experiment, and the transesterification conditions were optimized by orthogonal experiment. The results showed that the transesterification conversion rate reached 85.35% under the conditions of methanol dosage 45% (based on the mass of rapeseed oil deodorized distillate), system pH 7.0, reaction temperature 55 ℃ and reaction time 16 h. The study indicated that lipase EC 3.1.1.3 could be used to catalyze the transesterification of rapeseed oil deodorized distillate, which provided a new way to achieve cleaner production. |
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