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| 油酰乙醇酰胺改善LO2细胞中脂肪酸诱导的氧化应激 |
| Ameliorates of oleoylethanolamide on fatty acid-induced oxidative stress in LO2 cells |
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| DOI：10.12166/j.zgyz.1003-7969/2020.10.018 |
| 中文关键词: 油酰乙醇酰胺 脂肪酸 LO2细胞 氧化应激 非酒精性脂肪肝 |
| 英文关键词:oleylethanolamide fatty acid LO2 cell oxidative stress nonalcoholic fatty liver |
| 基金项目: |
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| 中文摘要: |
| 非酒精性脂肪性肝病（NAFLD）是最常见的慢性疾病之一，氧化应激在其发病和发展过程中起着关键作用。油酰乙醇酰胺（OEA）作为一种天然存在的脂质信号分子，具有多种药理学特性。研究探讨了油酰乙醇酰胺对脂肪酸诱导的LO2细胞氧化应激的影响及作用机制。通过0.4 mmol/L油酸和棕榈酸混合物（摩尔比2∶ 1）诱导LO2细胞产生氧化应激，添加不同浓度的OEA共同处理24 h。结果表明，OEA能够显著降低LO2细胞内ROS和MDA含量，提高抗氧化酶（SOD、CAT和GPx）活性。进一步的研究表明，OEA主要通过上调Nrf2和HO-1蛋白及mRNA的表达水平来改善氧化应激。 |
| 英文摘要: |
| Nonalcoholic fatty liver disease (NAFLD) is considered as one of the most common chronic disease, and oxidative stress plays an important role in its pathogenesis and development. Oleoylethanolamide(OEA), a naturally occurring lipid signaling molecule, has a variety of pharmacological properties. The effect and mechanism of oleylethanolamide on oxidative stress in fatty acid-induced LO2 cells were investigated. The cells were induced by 0.4 mmol/L mixture of oleic acid and palmitic acid (molar ratio 2∶ 1), and then treated with different concentrations of OEA for 24 h. The results showed that OEA significantly reduced the contents of reactive oxygen species (ROS) and malondialdehyde (MDA) in LO2 cells and increased the activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). Further research indicated that OEA increased the expressions level of nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase 1 (HO-1) protein and mRNA to ameliorate oxidative stress. |
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