| The method of monitoring glycerolysis of oil-tea camellia seed oil by nuclear magnetic resonance (NMR)was studied. The characteristic indexes of monoacylglycerols (MAG), diacylglycerols (DAG) and triacylglycerols (TAG) were searched from NMR spectrum, and the quantitative relationship between the characteristic index and content was established so as to effectively monitor the reaction degree of glycerolysis of oil-tea camellia seed oil. The chemical shifts of the characteristic peaks of monoacylglycerols, 1,2-diacylglycerols, 1,3-diacylglycerols and triacylglycerols were δ 392, δ 509, δ 4.09 and δ 5.27, and their relative peak areas were 88.69,42.11,27.26 and 36.74, respectively. Then the calculation method of glyceride content in unknown samples was deduced. It was found that there was no significant difference between the results of NMR and column chromatography in monitoring glycerolysis of oil-tea camellia seed oil by comparison. The results obtained by NMR were reliable and NMR could be used for monitoring the glycerolysis of oil-tea camellia seed oil. |
