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| Compositions of silkworm pupa oil and protein and potential allergen distribution |
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| DOI：10.12166/j.zgyz.1003-7969/2020.01.008 |
| KeyWord:silkworm pupa silkworm pupa oil silkworm pupa protein allergen |
| FundProject:“十三五”国家重点研发计划重点专项（2016YFD0400200）； 国家自然科学基金面上项目（31671881）； 重庆市基础科学与前沿技术研究项目（cstc2018jcyjA0939）； 中央高校基本科研业务费重点项目（XDJK2019B028） |
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| Abstract: |
| To further strengthen the comprehensive utilization of silkworm pupa, using fresh silkworm pupa as raw material, the fatty acid composition of silkworm pupa oil and the isoelectric point, relative content and subunit composition of silkworm pupa proteins with different solubility characteristics were studied. Furthermore, the potential allergen distributions in different proteins were also analyzed. The results showed that the relative content of unsaturated fatty acids in silkworm pupa oil was 60.83%, in which the content of oleic acid was the highest (35.43%). The relative content of insoluble protein in silkworm pupa protein was the highest (53.00%), showing a subunit distribution in 27-85 kDa; the water-soluble protein accounted for 23.63% and the isoelectric point was 3.8, showing a subunit distribution in 25-85 kDa; the alkali-soluble protein accounted for 19.73% and the isoelectric point was 44, showing a subunit distribution in 13-29 kDa; the salt-soluble protein accounted for 2.58% and the isoelectric point was 2.0, showing a subunit distribution in 15-35 kDa; the alcohol-soluble protein only accounted for 1.06% and the isoelectric point was 4.2, showing subunits distribution mainly in about 12 kDa and 20 kDa. Allergen distribution studies showed that the water-soluble, alcohol-soluble, salt-soluble,alkali-soluble and insoluble protein contained six, eight, eight, twelve and three kinds of allergens respectively, and their relative contents were 1.33%,2564%,1.21%,17.93% and 9.86% respectively. Proteins with high levels of allergens must be desensitized before being used in the food industry. |
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