Optimization of enzymatic transesterification to prepare diglyceride
  
DOI:10.12166/j.zgyz.1003-7969/2020.01.012
KeyWord:diglyceride  soybean oil  monoglyceride  transesterification  immobilized lipase
FundProject:“十三五”国家重点研发计划(2016YFD0401404);河南省科技厅自然科学项目(152100210265)
Author NameAffiliation
MA Jingxuan MA Chuanguo LI Lijun LIU Wei LIU Jun 1.College of Food Science and TechnologyHenan University of TechnologyZhengzhou 450001China
2.National Engineering Laboratory for Wheat and Corn Further ProcessingZhengzhou 450001China
3.Grain and Oil Food Engineering Technology Research Center,State Administration of Grain, Zhengzhou 450001,China 
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Abstract:
      Diglyceride was synthesized from soybean oil and monoglyceride by immobilized Novozym 435 in a solvent-free system. The effects of reaction temperature, reaction time, enzyme dosage, and substrate molar ratio on the diglyceride content were studied by single factor experiment and response surface methodology. The results showed that the effect of reaction temperature on the diglyceride content was the greatest, followed by reaction time, enzyme dosage and substrate molar ratio. The optimal conditions were obtained as follows: molar ratio of soybean oil to monoglyceride 1∶ 2, enzyme dosage 9%, reaction temperature 83 ℃ and reaction time 6.5 h. Under the optimal conditions, the diglyceride content was (51.2±0.2)%.
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