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Effect of refining process on triterpenes content in oil-tea camellia seed oil |
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DOI:10.12166/j.zgyz.1003-7969/2020.11.005 |
KeyWord:oil-tea camellia seed oil triterpene refining process |
FundProject:江西省重点研发项目(20192BBF60047,20192ACB60006);国家市场监督管理总局项目(2019MK040);江西省市场监督管理局项目(GSJK201916) |
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Abstract: |
Oil-tea camellia seed oils from different refining processes were separated by neutral alumina column chromatography with n-hexane as eluent, then GC-MS was used to determine the contents of eleven triterpenes in oil-tea camellia seed oil in each process to study the effect of refining process on triterpenes content in oil-tea camellia seed oil. The results showed that cycloartenol and β-amyrin were the main triterpenes in oil-tea camellia seed oil, and their contents in crude oil reached 1 043.30 mg/kg and 878.24 mg/kg respectively.The contents of triterpenes reduced after deacidification, bleaching and deodorization except lupeol,in which β-sitosterol and β-amyrin contents were greatly affected by deacidification, squalene content was greatly affected by bleaching, and cycloartenol content was greatly affected by bleaching and deodorization. No brassicasterol, uvaol and betulin were detected in oil-tea camellia seed oil in each refining process.Through the study, the main triterpene types in oil-tea camellia seed oil were initially identified, and data support was provided for the proper refining of oil-tea camellia seed oil. |
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