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| Determination of aflatoxin B1 in rapeseed oil by low temperature method |
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| DOI：10.12166/j.zgyz.1003-7969/2020.01.011 |
| KeyWord:rapeseed oil aflatoxin B1 low temperature detection HPLC |
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| Abstract: |
| According to the GB 5009.22—2016 National Food Safety Standard for the Determination of Aflatoxin B and G in Foods, the second method for detecting aflatoxin B1 in rapeseed oil has many problems in getting miscellaneous peaks, uncertainty and quantification. The method optimized the extraction reagent based on the second method of GB 5009.22—2016, and reduced the solubility of the impurities in the rapeseed oil by reducing the temperature of the extractant to achieve an ideal purification effect.Methanol-acetonitrile (volume ratio 70∶ 30) placed in a refrigerator at 4 ℃ for 30 min was added to the sample. After shaking, the supernate was blown to near dryness and then derivatized, and then dissolved with initial mobile phase and injected to high performance liquid chromatography for detection. A Waters XBridge BEH C18 column (4.6 mm × 100 mm, 2.5 μm) was used with a gradient elution of distilled water and acetonitrile as the mobile phase. The flow rate was 0.8 mL/min, the column temperature was 40 ℃, and the injection volume was 10 μL for qualitative and quantitative analysis. Under the optimal conditions, the quantitative linear range,the limit of quantification, the recovery rate and the precision of the method were 0.1-4.0 ng/mL, 0.1 μg/kg, 78.0%-90.4% and 1.61%-4.15% respectively. Compared with the national standard, the method could effectively remove impurities from the rapeseed oil, accurately conduct qualitative and quantitative analysis, and had good recovery rate and accuracy, and could be applied to the daily detection of aflatoxin B1 in rapeseed oil. |
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