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| Properties and identification of endogenous proteases in crude sunflower seed oil body |
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| DOI：10.12166/j.zgyz.1003-7969/2020.11.014 |
| KeyWord:sunflower seed crude oil body endogenous protease |
| FundProject:国家自然科学青年基金（31301496） |
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| Abstract: |
| The hydrolytic properties of endogenous proteases in crude sunflower seed oil body were analyzed by Tricine-SDS-PAGE, and the types of endogenous proteases were identified by LC-MS/MS. The results showed that the hydrolysis rate of oleosins was relatively faster at pH 4-8. 22 kDa globulin was rapidly hydrolyzed at pH 3-5, and 35 kDa globulin was rapidly hydrolyzed at pH 4-8. The hydrolysis rates of endogenous proteases in crude sunflower seed oil body were relatively the fastest at pH 4 and 50 ℃. The results of inhibitor experiments showed that at pH 4, the activities of metalloproteinases, aspartic protease and serine proteases were characterized in crude sunflower seed oil body, and there was almost no cysteine protease activity. LC-MS/MS analysis revealed that the metalloproteinases, aspartic protease and serine proteases, and aspartic proteases inhibitor, serine proteases inhibitor and cysteine protease inhibitor were presented in crude sunflower seed oil body. The research results provided a theoretical basis for the use of endogenous proteases for aqueous processing. |
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