A fast method for the determination of benzo(a)pyrene content in vegetable oil
  
DOI:10.12166/j.zgyz.1003-7969/2020.11.015
KeyWord:benzo(a)pyrene  vegetable oil  fast detection  HPLC
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Author NameAffiliation
HUANG Yuan National Quality Supervision and Testing Center for Processed Food, Fujian Inspection and Research Institute for Product Quality, Fuzhou 350001, China 
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Abstract:
      A fast, efficient and sensitive method for the determination of benzo(a)pyrene (BaP) in vegetable oil was established.The tetrahydrofuran was taken as extraction solvent, and sample solution was injected into the HPLC directly after filtering by 0.22 μm filter membrane.The results showed that the method had good linearity in the range of 0.1-20 ng/mL for BaP, with the correlation coefficient (R2) of 0.999 9, and the detection limit and quantitation limit were 0.1 μg/kg and 0.3 μg/kg, respectively, the recovery rate of real sample spiked was 93.5%-102.5%.This proposed method had been successfully applied to analyze BaP in six kinds of vegetable oils with good reproducibility and high sensitivity, and the method had a great practical application value.
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