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| Detection of six mycotoxins in edible vegetable oil by high performance |
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| DOI：10.12166/j.zgyz.1003-7969/2020.11.016 |
| KeyWord:mycotoxins high performance liquid chromatography liquid-liquid extraction technology edible vegetable oil |
| FundProject:“十三五”国家重点研发计划（2018YFC1604206）；国家自然科学基金（31772069） |
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| Abstract: |
| A method for simultaneous determination of six mycotoxins in edible vegetable oil by high performance liquid chromatography(HPLC) based on dispersion liquid-liquid microextraction technology (DLLME) was established. The sample was defatted with petroleum ether, ultrasound-extracted with acetonitrile-water-acetic acid (volume ratio 84∶ 15∶ 1), purified by liquid-liquid extraction with CHCl3 as extractant and pre-column derived.Then, it was separated by Agilent XDB-C18 chromatographic column (4.6 mm×150 mm, 3.5 μm) with gradient elution, and detected by fluorescence detector.The results showed that the linear relationships of the six mycotoxins in edible vegetable oil were good and the correlation coefficients were all more than 0.999.The detection limit of the method was 0.2-0.5 μg/kg.The average recovery of the samples was 75.88%-105.25%, and the relative standard deviation was 0.5%-9.5%.This method could be used for the simultaneous detection of six mycotoxins including aflatoxins (AFB1,AFB2,AFG1,AFG2) and ochratoxin (OTA, OTB) in edible vegetable oil. |
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