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| Determination of stigmastadiene and fatty acid alkyl esters in commercial olive oil |
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| DOI：10.12166/j.zgyz.1003-7969/2020.11.021 |
| KeyWord:olive oil stigmastadiene fatty acid alkyl esters quality adulteration |
| FundProject:湖北省食品药品监督管理局2018—2019年度科研项目（201802009） |
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| Abstract: |
| A method with simple and rapid pretreatment for the determination of stigmasterodiene content in olive oil was established, and combining with the determination of fatty acid alkyl esters in olive oil, the commercial olive oil samples of different grades were analyzed. The results showed that the sample was dissolved with n-hexane after adding the internal standard, and cleaned up on a commercial silica gel column. And then it was separated by DB-5MS chromatographic column and analyzed by GC-MS in selected ion monitoring mode to determine the stigmasterodiene content. The detection limit and quantification limit of stigmasterodiene were 0.05, 0.10 mg/kg, and there was a good linear relationship in the range of 0.05-2.0 μg/mL. The rate of recovery was 98.05%-100.19%, with RSD of 0.54%-3.04%. The contents of stigmadiene and fatty acid alkyl esters had significant differences in different grades olive oils, and the combined detection of them had important reference value in the identification of the quality and adulteration of extra virgin olive oil. |
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