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| Preparation of ACE inhibitory peptide from hazelnut meal by enzymatic hydrolysis |
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| DOI：10.12166/j.zgyz.1003-7969/2020.01.015 |
| KeyWord:hazelnut meal ACE inhibitory peptide enzymatic hydrolysis neutral protease ACE inhibition rate |
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| Abstract: |
| The hazelnut meal was hydrolyzed by neutral protease to prepare ACE inhibitory peptide. Taking the ACE inhibition rate of the enzymatic hydrolysates as an index, single factor experiment was carried out to investigate the effects of substrate mass fraction, enzyme dosage, enzymatic hydrolysis temperature, pH and enzymatic hydrolysis time. On the basis of single factor experiment, response surface Box-Behnken central combination experiment was designed to optimize the enzymatic hydrolysis conditions. The results showed that the optimal enzymatic hydrolysis conditions were obtained as follows: substrate mass fraction 5%, enzyme dosage 0.3%, enzymatic hydrolysis temperature 40 ℃, pH 7.5 and enzymatic hydrolysis time 1.5 h. Under these conditions, the ACE inhibition rate of enzymatic hydrolysates could reach 91.76%. |
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