|
| Determination of 17 amino acids in cottonseed protein by pre-column derivatization coupled with high performance liquid chromatography |
| |
| DOI：10.12166/j.zgyz.1003-7969/2020.12.004 |
| KeyWord:cottonseed protein amino acid high performance liquid chromatography 2,4-dinitrochlorobenzene derivatization |
| FundProject: |
|
| Hits: 1163 |
| Download times: 571 |
| Abstract: |
| A method for the determination of 17 amino acids in cottonseed protein by high performance liquid chromatography (HPLC) was developed. The sample was derived with 2,4-dinitrochlorobenzene (CDNB), separated by C18 column and detected by ultraviolet detector with detection wavelength 360 nm. The acid hydrolysis method, derivatization mode and reaction time were optimized. In view of the long sample pretreatment steps and many factors affecting the detection precision, the precision of each step was validated by backward method. At the same time, the internal standard method was used to quantify, which eliminated the influence of uncertain factors on the detection results in the detection process, and improved the detection precision. The results showed that 17 kinds of amino acids showed good linear relationship in the range of 0.004 6-0.045 7 mg/mL, the correlation coefficient was 0.983 2-0.999 8; the precision and the recovery rate were 1.71%-8.43% and 90.12%-109.67% respectiveyly except methionine and cystine. |
| View full text View/Add Comment Download reader |
| Close |
|
|
|
