With H. rhamnoides subsp. sinensis and H. rhamnoides subsp. turkestanica berry as raw materials, sea buckthorn berry oils were extracted by solvent method and their physicochemical properties and active components were analyzed. Combined with the principal component analysis (PCA) method, the classification model was established, and the differential components were determined.The results showed that the oil content of H. rhamnoides subsp. turkestanica berry was higher than that of H. rhamnoides subsp. sinensis berry. There were significant differences between the total phenol content, the total carotenoid content, peroxide value, iodine value, saponification value, acid value and color in H. rhamnoides subsp. sinensis berry oil and H. rhamnoides subsp. turkestanica berry oil. The main fatty acids of H. rhamnoides subsp. sinensis berry oil were palmitic acid, stearic acid, oleic acid and linoleic acid. The main fatty acids of H. rhamnoides subsp. turkestanica berry oil were palmitic acid, palmitoleic acid, oleic acid, linoleic acid and linolenic acid. The highest phytosterol content was β-sitosterol. GC-MS/PCA could intuitively distinguish different subspecies of H. rhamnoides subsp. sinensis and H. rhamnoides subsp. turkestanica, which could provide a reference for classification, quality control and authenticity recognition of sea buckthorn. |