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| 琼崖海棠籽油的遗传毒性评价 |
| Evaluation of genotoxicity of Calophyllum inophyllum seed oil |
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| DOI：10.12166/j.zgyz.1003-7969/2020.12.013 |
| 中文关键词: 琼崖海棠籽油 骨髓红细胞微核试验 精子畸形试验 Ames试验 遗传毒性 |
| 英文关键词:Calophyllum inophyllum seed oil bone marrow erythrocyte micronucleus test sperm malformation test Ames test genotoxicity |
| 基金项目:海南省创新能力建设计划-省属科研院所技术开发专项（KYYS-2018-34） |
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| 中文摘要: |
| 通过小鼠骨髓红细胞微核试验、精子畸形试验和Ames试验，对琼崖海棠籽毛油的遗传毒性进行了研究。结果表明：琼崖海棠籽毛油小鼠骨髓红细胞微核试验、精子畸形试验的中（1/4 LD50）、高（1/2 LD50）剂量组均较阴性对照组具有极显著差异（P<0.01），且呈剂量-反应关系，为阳性结果；Ames试验各剂量组均为阴性结果。琼崖海棠籽毛油中所含呫吨酮类、香豆素类、萜类和生物碱等化学物质可能是遗传毒性为阳性的原因。小鼠骨髓红细胞微核试验、精子畸形试验阴性结果剂量明显高于体重50 kg成年人的日推荐油脂摄入量（500~600 mg/kg）。因此，在推荐油脂摄入量下，琼崖海棠籽毛油对受试物不具有遗传毒性。 |
| 英文摘要: |
| Through the bone marrow erythrocyte micronucleus test, sperm malformation test in mice and Ames test, the genotoxicity of crude Calophyllum inophyllum seed oil was studied. The results showed that compared with the negative control group, the medium (1/4 LD50) and high (1/2 LD50) dose groups of crude Calophyllum inophyllum seed oil showed extremely significant differences in the bone marrow erythrocyte micronucleus test and sperm malformation test, and showed dose-dependant relationship, indicating positive result. The Ames test was negative for all dose groups. The presence of xanthones, coumarins, terpenoids and alkaloids in the oil might be the reason for the positive result of the genotoxicity. The doses of negative results of bone marrow erythrocyte micronucleus test and sperm malformation test in mice were obviously higher than the daily recommended oil intake dose of 500-600 mg/kg for adults weighing 50 kg. So the crude Calophyllum inophyllum seed oil was not genotoxic to the tested mice under the recommended oil intake. |
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