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| A feasibility study on predicting the toxicity of edible oils using zebrafish embryos |
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| DOI：10.12166/j.zgyz.1003-7969/2020.12.014 |
| KeyWord:edible oil zebrafish embryo acute toxicity test security non-target chemical analysis |
| FundProject:香港特别行政区企业支援计划（S/E035/15） |
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| Abstract: |
| The feasibility of predicting the toxicity of edible oils using zebrafish embryos was investigated. Seven lards with different qualities were collected from Taiwan, China and were extracted with acetonitrile and n-hexane, and then dissolved with methanol. The acute toxicity of the lard extracts was assessed using zebrafish embryos. The potential toxic component in the representative low, moderate, and high toxicity samples were further analyzed using non-target high-resolution Hybrid Quadrupole-Orbitrap mass spectrometer coupled on-line to a UHPLC/MS system. The structure and function of the potential toxic components in lard sample were predicted using ChemSpider, CT-link and Vega softwares. The results showed that the acute toxicity of the lard with different qualities were very different, and the potential toxic compounds in the high toxicity lard were identified to be oxidized fatty acid. Zebrafish embryo acute toxicity test could well predict the quality of different lards.The method provided a new idea to assess the safety of edible oils. |
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