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| 山茶油中不同形态酚类化合物的分离提取及抗氧化能力评价 |
| Extraction method of phenolic compounds in different forms from Camellia oleifera oil and its antioxidant ability |
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| DOI：10.12166/j.zgyz.1003-7969/2020.12.017 |
| 中文关键词: 山茶油 酚类化合物 提取方法 抗氧化能力 |
| 英文关键词:Camellia oleifera oil phenolic compound extraction method antioxidant ability |
| 基金项目: |
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| 中文摘要: |
| 以山茶油为原料提取4种形态酚类化合物（游离态、酯化、糖苷态、不溶性结合态），通过单因素实验优化提取条件，测定了不同形态酚类化合物总酚、总黄酮和缩合单宁含量，并采用DPPH、ABTS、FRAC和ORACFL4种方法评价不同形态酚类化合物抗氧化能力的差异。结果表明：山茶油中酯化、糖苷态及不溶性结合态酚类化合物的最佳水解时间分别为4、1、4 h，最佳液料比（体积比）分别为2∶ 1、1∶ 1、4∶ 1；山茶油中总酚含量（TPC）为（225.74±0.10）μg/g，主要以糖苷态存在，占总TPC的57.02%；山茶油中4种形态酚类化合物抗氧化能力的强弱顺序为糖苷态酚类化合物>不溶性结合态酚类化合物>酯化酚类化合物>游离态酚类化合物。 |
| 英文摘要: |
| Four forms of phenolic compounds (free, esterified, glycosidic, insoluble-bound) were extracted from Camellia oleifera oil. The extraction conditions were optimized by single factor experiment. The contents of total phenols, total flavonoids and condensed tannins of four forms of phenolic compounds in Camellia oleifera oil were determined. DPPH, ABTS, FRAC and ORACFL were used to compare the antioxidant ability of four forms of phenolic compounds in Camellia oleifera oil. The results showed that the optimal hydrolysis time of esterified, glycosidic and insoluble-bound phenolic compounds in Camellia oleifera oil was 4, 1 h and 4 h respectively, while the best liquid-material ratios (volume ratios) for esterified, glycosidic and insoluble-bound phenolic compounds in Camellia oleifera oil were 2∶ 1, 1∶ 1 and 4∶ 1. The total phenol content (TPC) in Camellia oleifera oil was (225.74±0.10）μg/g. And the main form of phenolic compounds in Camellia oleifera oil was glycosidic, accounting for 57.02% of total TPC. The order of the antioxidant ability of four forms of phenolic compounds from high to low was glycosidic, insoluble-bound, esterified, free forms. |
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