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| Extraction method of phenolic compounds in different forms from Camellia oleifera oil and its antioxidant ability |
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| DOI：10.12166/j.zgyz.1003-7969/2020.12.017 |
| KeyWord:Camellia oleifera oil phenolic compound extraction method antioxidant ability |
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| Abstract: |
| Four forms of phenolic compounds (free, esterified, glycosidic, insoluble-bound) were extracted from Camellia oleifera oil. The extraction conditions were optimized by single factor experiment. The contents of total phenols, total flavonoids and condensed tannins of four forms of phenolic compounds in Camellia oleifera oil were determined. DPPH, ABTS, FRAC and ORACFL were used to compare the antioxidant ability of four forms of phenolic compounds in Camellia oleifera oil. The results showed that the optimal hydrolysis time of esterified, glycosidic and insoluble-bound phenolic compounds in Camellia oleifera oil was 4, 1 h and 4 h respectively, while the best liquid-material ratios (volume ratios) for esterified, glycosidic and insoluble-bound phenolic compounds in Camellia oleifera oil were 2∶ 1, 1∶ 1 and 4∶ 1. The total phenol content (TPC) in Camellia oleifera oil was (225.74±0.10）μg/g. And the main form of phenolic compounds in Camellia oleifera oil was glycosidic, accounting for 57.02% of total TPC. The order of the antioxidant ability of four forms of phenolic compounds from high to low was glycosidic, insoluble-bound, esterified, free forms. |
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