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| Extraction of polysaccharides from oil peony seed meal using a thermosensitivedeep eutectic solvents-aqueous two-phase system |
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| DOI：10.12166/j.zgyz.1003-7969/2020.12.018 |
| KeyWord:deep eutectic solvents polysaccharides oil peony seed meal temperature induced aqueous two-phase |
| FundProject:甘肃省高校协同创新科技团队支持计划(2017C-17) |
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| Abstract: |
| A thermosensitive deep eutectic solvents (DES)-aqueous two-phase system (ATPS) was developed for the extraction and preliminary purification of polysaccharides in oil peony seed meal,and the properties of the polysaccharides were determined. Using 16 kinds of DES as extractants to prepare crude polysaccharides from oil peony seed meal, and DES8(ChCl-EG) was preferred as an extractant to extract polysaccharides,and the yield of polysaccharides was 96.58 mg/g under the conditions of water content of DES8 50%, ratio of raw material to water 1∶ 30, extraction temperature 50 ℃, and extraction time 40 min. Then the crude polysaccharides were extracted and preliminarily purified by EOPO/DES-ATPS. When the final mass fraction of EOPO2500 in solution was 55% and the extraction temperature was 45 ℃, the maximum extraction rate 89.56% was obtained and the recovery rate of polysaccharides was 86.15% after temperature induced separation of EOPO phase rich in polysaccharides. The polysaccharides prepared had a major relative molecular weight of 67.76 kDa, and comprised of arabinose, mannose and glucose with molar ratio of 1∶ 2.5∶ 078. The aqueous two-phase system based on thermal separation had the advantages of friendly environment, simple operation, convenient use and easy recovery of components, and could be used as an effective method for polysaccharides extraction and separation. |
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