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| 温敏性低共熔溶剂双水相萃取分离油用牡丹籽粕多糖 |
| Extraction of polysaccharides from oil peony seed meal using a thermosensitivedeep eutectic solvents-aqueous two-phase system |
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| DOI：10.12166/j.zgyz.1003-7969/2020.12.018 |
| 中文关键词: 低共熔溶剂 多糖 油用牡丹籽粕 温度诱导双水相 |
| 英文关键词:deep eutectic solvents polysaccharides oil peony seed meal temperature induced aqueous two-phase |
| 基金项目:甘肃省高校协同创新科技团队支持计划(2017C-17) |
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| 中文摘要: |
| 采用温敏性低共熔溶剂（DES）双水相萃取分离油用牡丹籽粕多糖，并对其性质进行了研究。以16种DES为提取剂制备油用牡丹籽粕多糖粗提物，优选出DES8（氯化胆碱-乙二醇）作为提取剂，在其含水量50%、固液比1∶ 30、提取温度50 ℃、提取时间40 min时，多糖得率最高，为96.58 mg/g；采用EOPO/DES双水相体系对多糖提取物进行萃取，当EOPO2500在溶液中终质量分数为55%、萃取温度为45 ℃时，多糖萃取率最大，为89.56%；将富含多糖的EOPO相进行温度诱导分离，多糖回收率为86.15%。低共熔溶剂制备的多糖相对分子质量主要为67.76 kDa，其由阿拉伯糖、甘露糖和葡萄糖组成，摩尔比为1∶ 2.5∶ 0.78。基于热分离的双水相体系具有环境友好、操作简单、使用方便及组分易回收等优点，可作为多糖萃取分离的有效方法。 |
| 英文摘要: |
| A thermosensitive deep eutectic solvents (DES)-aqueous two-phase system (ATPS) was developed for the extraction and preliminary purification of polysaccharides in oil peony seed meal,and the properties of the polysaccharides were determined. Using 16 kinds of DES as extractants to prepare crude polysaccharides from oil peony seed meal, and DES8(ChCl-EG) was preferred as an extractant to extract polysaccharides,and the yield of polysaccharides was 96.58 mg/g under the conditions of water content of DES8 50%, ratio of raw material to water 1∶ 30, extraction temperature 50 ℃, and extraction time 40 min. Then the crude polysaccharides were extracted and preliminarily purified by EOPO/DES-ATPS. When the final mass fraction of EOPO2500 in solution was 55% and the extraction temperature was 45 ℃, the maximum extraction rate 89.56% was obtained and the recovery rate of polysaccharides was 86.15% after temperature induced separation of EOPO phase rich in polysaccharides. The polysaccharides prepared had a major relative molecular weight of 67.76 kDa, and comprised of arabinose, mannose and glucose with molar ratio of 1∶ 2.5∶ 078. The aqueous two-phase system based on thermal separation had the advantages of friendly environment, simple operation, convenient use and easy recovery of components, and could be used as an effective method for polysaccharides extraction and separation. |
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